Glycoproteomics and Protein Expression
Glycoproteomics is a special branch of
proteomics that identifies, catalogs, and characterizes carbohydrate containing
proteins after the completion of post-translational modification. Protein
expression is the process in which proteins synthesis, regulation and
modification occurs in living organisms. Analysis of wide range of structurally
diverse proteins by genomic technologies and due to increasing complexity in
disease targets, innovative approaches for the purification, expression and
characterisation of recombinant proteins has increased over years.
Protein expression is the biotechnological
procedure of generating a specific protein. It is achieved by the manipulation
of gene expression in an organism such that it expresses large amounts of a
recombinant gene. This contains the transcription of the recombinant DNA to
messenger RNA (mRNA), the translation of mRNA into polypeptide chains, which
are ultimately folded into functional proteins and may be targeted to exact
subcellular or extracellular locations.